Saturday, January 5, 2019

1.8 explain how information from chromatograms can be used to identify the composition of a mixture.



The unknown mixture must be placed alongside known dyes on the same piece of paper and the chromatogram run with a suitable solvent.  The separated spots of colour in the mixture are then matched to the spots of known dyes – they will travel the same distance up the chromatography paper.
Sometimes we calculate a quantity known as the retention factor (Rf).  To do this, you measure the distance travelled by the solvent from the base line and the distance travelled by the spot of colour from the base line.
Rf  =     distance moved by coloured spot
            distance moved by solvent

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