The unknown mixture must be placed alongside
known dyes on the same piece of paper and the chromatogram run with a suitable
solvent. The separated spots of colour
in the mixture are then matched to the spots of known dyes – they will travel
the same distance up the chromatography paper.
Sometimes we calculate a quantity known as the
retention factor (Rf). To do
this, you measure the distance travelled by the solvent from the base line and
the distance travelled by the spot of colour from the base line.
Rf = distance moved by coloured spot
distance moved by
solvent
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